鳙内源性转谷氨酰胺酶特性的研究
Characteristics of transglutaminase from bighead carp
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摘要: 文章研究了温度、pH、二硫苏糖醇(DTT)以及不同的金属离子和抑制剂对鳙(Hypophthalmichthys nobilis)鱼肉中内源性转谷氨酰胺酶(transglutaminase,TGase)活性的影响。结果表明,鳙内源性TGase的最适温度在37~45 ℃之间,最适pH为6.5~7.5,适量的钙离子(Ca2+)(0~2.2 mmolL-1)可以激发酶活性,低浓度(0~0.2 mmolL-1)的DTT可以提高酶活性,但高浓度的钠离子(Na+)和钾离子(K+)会降低酶活性,镁离子(Mg2+)、铜离子(Cu2+)、锌离子(Zn2+)、钡离子(Ba2+)、铁离子(Fe3+)、亚铁离子(Fe2+)、铅离子(Pb2+)和乙二胺四乙酸(EDTA)也会抑制酶的活性。Abstract: We studied the transglutaminase (TGase) from bighead carp (Hypophthalmichthys nobilis) under different conditions (temperature, pH, DTT). The optimum temperature and pH of TGase were 37~45 ℃ and 6.5~7.5, respectively. The optimum concentrations of CaCl2 and dithiothreitol were 0~2.2 mmolL-1 and 0~0.2mmolL-1, respectively. The TGase activity decreased as concentrations of Na+ and K+ increased, and was inactivated by Mg2+, Cu2+,Zn2+, Ba2+,Fe3+,Fe2+,Pb2+ and EDTA.