Abstract: Cyclin B plays an important role in oocyte development. The recombinant lentiviral vector of Penaeus monodon Cyclin B (PmCyB) was constructed. The recombinant lentiviral vector plasmid and Packaging Plasmid Mix were co-transfected into 293T cells to obtain recombinant lentivirus. AfterCyclin B1 of Hela cells was interfered with siRNA, the recombinant lentivirus was transfected into these cells and its influence on the Hela cells proliferation was researched. The results show that Hela cells proliferation slowed down after siRNA interference, but renewed after being transfected by the packaged lentivirus particles containing PmCyB, which indicates that PmCyB gene has cell cycle activity and is important for regulation of cell proliferation and differentiation gene. The results also suggest that PmCyB might play an important role in ovarian development, as same as Homo sapienseCyclin B1 which is an ovarian development essential gene.