Abstract: Through microdilution method, we studied the inhibitory effects of different processed products of Atractylodes lancea and water extract from different parts of Psidium guajava on 7 strains from 6 bacterial species, including Vibrio coralliilyticus, V. harveyi, Photobacterium damselae subsp piscicida, V. parahaemolyticus, Morganella fulton, V. vulnificus. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for different strains were determined. Results show that different Chinese medicine processed products of Atractylodes and different parts of P. guajava have better antibacterial effect to V. coralliilyticus, V. harveyi and P. damselae subsp piscicida, and the MIC and MBC are 10-3 gmL-1 and 10-1gmL-1 respectively. Antibacterial effect of P. guajava is leavesfruitsbranches, and of A.lancea is bran frysalt fry unprocessedvinegar fry.