Abstract: Fish specimens, as important research resources, play an important role in the studies of taxonomy, fishery resources and so on, but they are easy for deformation and color fading after long time preservation in formaldehyde saturated solution, and the number of visitors is relatively limited by the space restriction of specimen museums. Thus, we constructed 3D fish specimen museum by 1) using Strata Foto 3D to mask fish specimen images, build wire frame mode and increase surface texture processing, etc.; 2) using Strata Live 3D to complete the expressions of light, measurement, background pattern, projection and to achieve the web publishing of 3D model of fish specimens; 3) using ASP technology to complete the dynamic access of the specimen information database and display the biological information of specimens; 4) using AutoCAD and 3ds Max to integrate single specimen and whole specimen museum into 3D fish specimen museum; 5) using Unity 3D as a development platform to achieve 3D virtual scene roaming and web publication.