湛江官渡近江牡蛎养殖水细菌群落的DGGE指纹分析

DGGE fingerprinting of bacterial community in aquaculture water of Crassostrea ariakensis from Guandu district, Zhanjiang city

    摘要
  • 摘要: 2011年3月~12月利用变性梯充凝胶电泳（denatured gradient gel elctrophoresis, DGGE）技术对湛江官渡地区近江特蛎（Crassostrea ariakensis)养殖水细菌群落花流水组成进行了监测研究。UPGAMA聚类分析显示3月~12月的样品细菌主要分属2大类群；多样性指数结果显示3月~7月细菌多样性高于8月~12月；系统发育分析显示牡蛎养殖区水体优势菌群主要由以下种群组成：未可培养的变形菌门（Proteobacteria）（、、、）、未可培养的拟杆菌门（Bacterioidetes）、未可培养的疣微菌门（Verrucomicrobiae bacterium）、未可培养的放线菌门（Actinobacteria）和未可培养的蓝细菌门（Cyanobacter bacterium）。最优势菌群为-变形菌纲和拟杆菌门的黄杆菌属（Flevobacterium）。

     

    Abstract: We had monitored and investigated the bacterial community composition in aquaculture water for Crassostrea ariakensis in Guandu of Zhanjiang by denaturing gradient gel electrophoresis (DGGE) from March to December,2011. UPCMAclustering analysis shows that there are 2 major populationsofbacterialspecies. Diversity index reveals that the diversities inMarch~Julywere higher than thoseinAugust~December. Phylogenetic analysis indicates that the dominant microbial populations in the water are uncultured Proteobacteria ( , , and ), uncultured Bacterioidetes, uncultured Verrucomicrobiae bacterium, uncultured Actinobacteria and uncultured Cyanobacter bacterium. Uncultured -proteobacterium and Flevobacterium of uncultured Bacteroidetes are the most dominant bacteria.c

     

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