5个紫菜属物种丝状体的微卫星初步遗传分析

Preliminary genetic analysis of conchocelis from 5 Porphyra species using microsatellite

摘要: 文章利用68对微卫星引物对坛紫菜(Porphyra haitanensis)、半叶紫菜(P.katadai)、条斑紫菜(P.yezoensis)、少精紫菜(P.oligospermatangia)和皱紫菜(P.crispata)5个紫菜属物种的丝状体进行初步的遗传分析，共筛选出20对高多态性适用引物，在10份紫菜丝状体样品扩增位点的多态性比例为95.24%，每对引物可扩增的等位基因位点数为1.22~4.05，平均为2.37；各样品之间的遗传多样性指数为0.254 6~0.899 4；不同物种的遗传距离为0.106 0~1.406 6，同一物种的丝状体材料优先聚类为一支，表明筛选出的微卫星引物具有良好的遗传分辨能力。

Abstract: We conducted a preliminary genetic analysis of conchocelis from 5 Porphyra species (P.haitanensis, P.katadai, P.yezoensis, P.oligospermatangia and P.crispate) by 68 microsatellite primer-pairs, and selected 20 microsatellite primer-pairs with high polymorphism. The polymorphism proportion among 10 Porphyra conchocelis reaches 95.24%, and the effective number of alleles for each primer is 1.22~4.05 (average 2.37). The genetic diversity index is 0.254 6~0.899 4 and the genetic distance between different species is 0.106 0~1.406 6. Conchocelis from the same species group into one cluster first, which reveals that the selected microsatellite primer-pairs have good genetic resolution power.