Abstract: The distribution and cryopreservation of alkaline phosphatase (AKP) and acid phosphatase (ACP) activities in Trachinotus ovatus were studied. The samples were kept at 4 ℃, -20 ℃ and -80 ℃ for 3 d, 7 d, 15 d and 30 d, respectively, to investigate the effect of different storage temperatures and time on AKP and ACP activities. The results show that AKP activity has been tested in different organs except stomach, and the order of AKP activity is: hindgut > pyloric caecum > kidney > midgut > liver > foregut > heart > muscle. ACP has been tested in different organs, and the order of ACP activity is: liver > muscle > hindgut > midgut > pyloric caecum > kidney > heart > foregut > stomach. The result of cryopreservation experiment indicates that both AKP and ACP activities obviously weaken as the storage time prolongs at 4 ℃ and -20 ℃, but remain steady at -80 ℃ within 30 d except for pyloric caecum.