Abstract: A comparative study was conducted on the activities of digestive enzymes in different digestive organs of adult and young Trachinotus ovatus.Results show that: 1) For adults, the order of protease activity from strong to weak is: stomach > foregut > midgut > pyloric caecum > hindgut > liver; amylase activity: foregut > hindgut > pyloric caecum > midgut > liver > stomach; lipase activity: foregut > midgut > hindgut > pyloric caecum > liver > stomach. 2) For young fish, the order of protease activity is: stomach > intestine > pyloric caecum > liver; amylase activity: intestine > pyloric caecum > liver > stomach; lipase activity: intestine > pyloric caecum > liver > stomach. 3)Both the protease and amylase activities in different digestive organs of adults are weaker than those in young fish, and there is significant difference in amylase activity in stomach and pyloric caecum between adult and young fish (P < 0.05).The lipase activity in stomach of young fish is stronger than that in adults but weaker than that in the other digestive organs of adults. The order of digestive enzyme activity in young fish is similar with adults.