Abstract: Holothuria scabra is an important tropical sea cucumber species in South China. Combining methods of thermal stimulation, dry treatment and powerful water jet, etc., we artificially induced the spawning and successfully bred H.scabra for the first time in China.During April~June, 2010, among 10 batches of 243 H.scabra bloodstocks, 5 batches of parent H.scabra succeeded in sperm releasing and spawning (spawning rate: 50%); 1 652.72×10⁴ eggs were produced and 1 226.74×10⁴ larvae were hatched out (hatching rate: 74.23%).At water temperature of 27.5~30.0 ℃, it took 32.5 h for the fertilized eggs to finish embryo development. We chose some larvae for 21-day rearing and obtained 19.43×10⁴ early juveniles (size: 0.1 cm) with a survival of 16.7%; after 32-day rearing, 2.46×10⁴ late juveniles (size: 0.3~0.5 cm) were obtained with a survival of 2.12%. The study is important for the future natural H.scabra population recovery in Beibu Gulf and its industrialized breeding in South China.