Abstract: The families were established based on 4 different populations of black tiger shrimp Penaeus monodon, among which 3 wild broodstocks were caught from Thailand (T), Africa (A) and Sanya (S) coastal waters, while the other was artificial bred shrimp (F). The 9 representative families whose development time was almost the same were experimented for 30 days to study their growth and feed utilization. The results showed that the survival rate of the hybrid generations whose mothers got genetic features of P. monodon caught from Sanya coastal water was significantly higher than the others' (P0.05), but the growth performance was very significantly poorer than the others (P0.01) (except for TT); the weight gain rate of AA was 2 times as much as SS, but it showed no significant regularity in the feed conversion ratio of the hybrid generations.