Abstract: The flesh of Pinctada martensii with black shell disease was processed by adopting methods such as wet digestion and Soxhlet extraction + steam distillation. The main 12 trace elements of the samples were determined by ICP-AES and the volatile components were identified by GC-MS. Compared with healthy shellfish, there was no significant difference in the content of trace elements except for Zn and Se (decrease rates were 8.54% and 10.98% respectively) in sick P.martensii. The sick shellfish contained not only some metal elements and a large quantity of benzene ring, but also 16 kinds of volatile components while healthy shellfish had 20.