不同生长阶段与养殖环境下黄唇鱼肝脏脂肪沉积特征及脂肪肝等级初步评价

Hepatic lipid deposition characteristics and preliminary fatty liver grading in Bahaba taipingensis at different growth stages and aquaculture environments

  • 摘要: 为探明不同养殖阶段黄唇鱼 (Bahaba taipingensis) 死亡个体肝脏颜色与脂肪积累的特征及相关性,本研究以4月龄幼鱼、14月龄幼鱼、14月龄同批次放流后误捕个体及36月龄成鱼为研究对象,测定形态学指标,采用油红O和HE染色观察肝脏组织学变化,并通过图像分析定量肝脏脂肪阳性率。结果表明,不同生长阶段黄唇鱼肝脏颜色存在显著差异,依据颜色深浅可将肝脏分为颜色正常组与异常组。组织学观察显示,颜色正常组肝细胞结构完整、脂滴细小且分布均匀;颜色异常组出现脂滴融合、细胞核偏移及肝血窦狭窄等病理特征。对肝脏颜色进行标准化色度分级后,受试者工作特征 (Receiver operating characteristic, ROC) 曲线分析表明,肝脏颜色与肝细胞脂滴最大直径均能有效区分正常肝脏与脂肪肝损伤 (曲线下面积AUC>0.7)。此外,野外放流个体的肝脏脂肪阳性率低于室内同批次个体。黄唇鱼死亡个体肝脏颜色与脂肪积累程度高度相关,深黄色、灰白色及土黄色肝脏脂质沉积较严重,深红色肝脏脂质沉积较轻。肝脏颜色可作为黄唇鱼脂肪肝现场初筛的简易指标,对黄唇鱼健康养殖与精准管理具有一定实践意义。

     

    Abstract: To explore the characteristics and correlation between liver color and fat accumulation in dead Bahaba taipingensis at different growth and culture stages, we took 4-month-old juveniles, 14-month-old juveniles, accidentally recaptured individuals of the same released batch, and 36-month-old adults as research subjects. We measured morphological indices, observed liver histological changes via Oil Red O and hematoxylin-eosin (HE) staining, and quantitatively anaylzed the hepatic fat-positive rate by image analysis. The results showed that liver color of B. taipingensis differed significantly at different growth stages, and the livers could be divided into normal and abnormal color groups according to color shade. Histological observation indicated that normal-color group presented intact hepatocyte structure with fine and uniformly distributed lipid droplets; the abnormal-color group showed typical pathological features including lipid droplet fusion, nuclear compression and displacement, and narrowed hepatic sinusoids. After standardized chromatic grading of liver color, receiver operating characteristic (ROC) curve analysis revealed that both liver color and maximum diameter of hepatocellular lipid droplet could effectively distinguish normal livers from fatty liver lesions (AUC>0.7). Moreover, field-released individuals exhibited a lower hepatic fat-positive rate than indoor-cultured ones from the same batch. Liver color was highly correlated with the degree of hepatic lipid accumulation in dead B. taipingensis individuals. Severe lipid deposition was observed in livers with deep yellow, grayish-white and khaki yellow color, while mild lipid accumulation was detected in deep red livers. In conclusoin, liver color can serve as a convenient on-site screening index for fatty liver in B. taipingensis, and has practical significance for the healthy culture and precise management of this species.

     

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