Abstract: To address the unclear motion response and unique single-point mooring characteristics of hybrid-structure aquaculture vessels under moored conditions, this paper adopts the panel method based on potential flow theory and the Morison equation to perform time-domain analysis and calculation of the aquaculture vessel in a moored state. We systematically analyzed the effects of the presence of net cages, variations in draft depth, as well as changes in environmental water depth on the motion response and mooring forces, so as to discuss the variation of mooring forces under different mooring chain parameters. The results show that the mooring force on the aquaculture vessel was the largest when wind, waves, and current were in the same direction. The mooring force of the aquaculture vessel with net cages was 1.35 times that of the vessel without net cages. The mooring force reached its maximum at a draft of 4 m, and decreased with increasing water depth. As the anchor chain length increased, the maximum mooring force exhibited a gradually decreasing trend. With increasing counterweight mass, the maximum mooring force first decreased and then increased. With increasing distance from the counterweight to the anchor point, the maximum mooring force first increased and then decreased.