Abstract: Phages, which specifically infect and lyse bacteria, are of significant value in the prevention and treatment of bacterial infections, as well as in the management of environmental microbiota. Vibrio parahaemolyticus, a prevalent pathogen in aquaculture, causes diseases in aquatic animals and frequently leads to foodborne illnesses in humans. In this study, we isolated a phage specific to V. parahaemolyticus (vB_VpS_BA27) from water samples collected from a diseased snail pond in a Babylonia areolata aquaculture facility on Donghai Island, Zhanjiang. Then we systematically analyzed the biological characteristics and genomic features of vB_VpS_BA27. The results show that vB_VpS_BA27 remained active across a broad pH range (3–11) and exhibited robust survival capabilities in low-temperature conditions (4–37 ℃). It demonstrated significant antibacterial activity with a burst size of 285.67 PFU·cell⁻¹. Genomic sequencing confirms that vB_VpS_BA27 was a lytic phage, with no detected antibiotic resistance or virulence genes. Phylogenetic analysis indicates that within the class Caudoviricetes, vB_VpS_BA27 was closely clustered with members of the genus Delepquintavirus and that, together with other related phages, they formed a new clade within this class. The study demonstrates that vB_VpS_BA27 exhibits the potential in controlling vibriosis in B. areolata, providing a novel approach for the biological control of diseases in B. areolata aquaculture.