Abstract:
In order to develop the artificial breeding technology of
Temnopleurus reevesii, and to provide useful information for its artificial breeding and application, we carried out a series of laboratory procedures including spawning induction, fertilization, incubation and larval rearing. We observed the developmental process from fertilized egg to juvenile, and measured the size of the fertilized eggs, embryos and larvae. The results indicate that
T. reevesii could be successfully induced to spawn by injection of 0.5 mol·L
−1 KCl solution, and the fertilization rates ranged from 87.4% to 95.7%, with an average of (91.6±2.4)%. Under the conditions including water temperatures of 24-25 ℃ and a larval rearing density of approximately 1 ind·mL
−1, the cleavage commenced after 45 min, the morula stage was reached by 3 h and 45 min, the blastula stage was reached by 6 h, the gastrula stage was reached by 12 h, the two-armed larva stage was reached by 22 h, the four-armed larva stage was reached by 1 d and 12 h, the six-armed larva stage was reached by 5 d and 16 h, and the eight-armed larva stage was reached by 9 d. The larvae metamorphosis into juvenile was happened around 32 d. Compared with other sea urchin species,
T. reevesii developed faster prior to the four-armed larva stage, followed by a slower pace post that stage.