Abstract: To understand the utilization of the habitat in the waters along the Hong Kong-Zhuhai-Macao Bridge by Indo-Pacific humpback dolphin (Sousa chinensis) and its potential migration pathways to cross the bridge. We set four fixed passive acoustic monitoring points (A, B, C, D) along the northern waters of the Hong Kong-Zhuhai-Macao Bridge, and recorded a total of 2 947 hours of acoustic data in November 2020 and March 2021, respectively. The probabilities of detecting acoustic events in S. chinensis at four monitoring points are as follows: 15.31% (A), 17.30% (B), 8.97% (C), 5.85% (D), which indicates that the waters along the northern of the bridge is an important habitat for S. chinensis with spatiotemporal differences. There is no significant diurnal and nocturnal difference in the acoustic events of S. chinensis observed at different monitoring sites. The probabilities of detecting S. chinensis at low tide levels of monitoring sites A, B and C on bridge structures were 1.56%, 1.82% and 0.78%, respectively, all higher than those at high tide levels (0.94%, 1.56% and 0.26%). The probabilities of discovering S. chinensis during high tide and low tide stages at sites A and B were similar, but those during high tide stage at sites C and D were 4.55% and 3.64%, both higher than those during low tide stage (3.12% and 1.30%), indicating that tidal changes have a certain impact on the habitat selection of S. chinensis in bridge structure waters with spatial differences. The result also indicates that S. chinensis may prefer the area between sites A and B to cross the bridge than the other area. Inspection and management of its passageway should be strengthened.