大泷六线鱼胃排空规律和摄食消化酶活力变化研究

Alternation of gastric emptying, feeding behavior and digestive enzyme activities in Hexagrammos otakii

  • 摘要: 为确定大泷六线鱼 (Hexagrammos otakii) 的最佳投喂间隔及次数,以平均体质量为 (73.19±6.68) g的大泷六线鱼为研究对象,统计摄食后不同时间的胃内容物占比,比较平方根模型、立方模型和线性模型对胃排空曲线的拟合程度,分析摄食后血清皮质醇、葡萄糖含量、肠道和肝脏消化酶活性的变化规律。结果显示,大泷六线鱼胃排空属于典型的直线下降型;且3种模型均能拟合其胃排空数据,但立方模型拟合最佳,并得出80% 胃排空约为摄食后15 h,基本排空 (97.7%) 约为18 h,完全排空约为19.7 h。血清皮质醇、葡萄糖含量、肠道和肝脏消化酶 (淀粉酶、糜蛋白酶和脂肪酶) 活性均在摄食后0~6 h显著上升,并在第6 小时达到峰值 (p<0.05),之后均显著下降。其中肠道淀粉酶活性在摄食后的第15小时降至最小值 (p<0.05);肝脏糜蛋白酶、肝脏脂肪酶活性和血清皮质醇含量则在摄食后的第18小时降至最小值;瞬时排空速率与葡萄糖、皮质醇含量和消化酶活性均呈正相关。综上,摄食后的第15 小时为大泷六线鱼胃排空和消化生理临界点,其最适投喂频率为每天2次。

     

    Abstract: In order to determine the optimal feeding interval and frequency for Hexagrammos otakii, taking H. otakii Average body mass of (73.19±6.68) g as research object, we observed the proportions of stomach contents at different time after feeding, then compared the fitting degrees of the square root model, cubic model and linear model on the gastric emptying curve, and analyzed the changes of serum cortisol and glucose contents, intestinal and liver digestive enzyme activities after feeding. The results show that the gastric emptying of H. otakii followed a typical linear descending pattern. All the three models could fit the gastric emptying data, especially the cubic model fitted the best. 80% gastric emptying took about 15 h after feeding; basic emptying (97.7%) took about 18 h; complete emptying took about 19.7 h. The serum cortisol, glucose level, and the activities of intestinal and hepatic digestive enzymes (Amylase, chymotrypsin, and lipase) increased significantly from 0–6 h after feeding, peaking at 6th hour (p<0.05), but then decreased significantly. The intestinal amylase decreased to the minimum values at 15th hour after feeding (p<0.05). The activities of liver chymotrypsin, liver lipase and serum cortisol levels decreased to the minimum values at 18th hour after feeding. The instantaneous emptying rate was positively correlated with the contents of glucose, cortisol and digestive enzyme activities. In conclusion, the physiological critical point of gastric emptying and digestion is 15 h after feeding in H. otakii, and the optimal feeding frequency for H. otakii is twice a day.

     

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