吴晓鹏, 黄敏伟, 陈晓瑛, 彭凯, 赵吉臣, 钟平, 刘凤坤, 张业辉, 黄文. 玉足海参变态附着阶段转录组分析[J]. 南方水产科学, 2023, 19(6): 84-96. DOI: 10.12131/20230105
引用本文: 吴晓鹏, 黄敏伟, 陈晓瑛, 彭凯, 赵吉臣, 钟平, 刘凤坤, 张业辉, 黄文. 玉足海参变态附着阶段转录组分析[J]. 南方水产科学, 2023, 19(6): 84-96. DOI: 10.12131/20230105
WU Xiaopeng, HUANG Minwei, CHEN Xiaoying, PENG Kai, ZHAO Jichen, ZHONG Ping, LIU Fengkun, ZHANG Yehui, HUANG Wen. Transcriptome analysis of metamorphosis stage of Holothuria leucospilota[J]. South China Fisheries Science, 2023, 19(6): 84-96. DOI: 10.12131/20230105
Citation: WU Xiaopeng, HUANG Minwei, CHEN Xiaoying, PENG Kai, ZHAO Jichen, ZHONG Ping, LIU Fengkun, ZHANG Yehui, HUANG Wen. Transcriptome analysis of metamorphosis stage of Holothuria leucospilota[J]. South China Fisheries Science, 2023, 19(6): 84-96. DOI: 10.12131/20230105

玉足海参变态附着阶段转录组分析

Transcriptome analysis of metamorphosis stage of Holothuria leucospilota

  • 摘要: 海参幼苗发育需要经历耳状幼体、樽形幼体、五触手幼体及幼苗阶段,而从浮游幼体变态发育至附着幼苗阶段的高死亡率是热带海参繁育中的共性问题,目前有关热带海参变态发育的调控机制仍不清楚。以玉足海参 (Holothuria leucospilota) 为研究对象,采集小耳幼体 (A)、中耳幼体 (B)、大耳幼体 (C) 和樽形幼体 (D) 4个时期样品进行高通量转录组测序分析,以探究其变态发育的分子机制。结果显示,共产生83.6 GB Raw reads,拼接获得93 528个Unigenes。对4个组测序文库的相邻组间 (A_vs_B, B_vs_C, C_vs_D) 进行两两比较,A_vs_B、B_vs_C和C_vs_D的差异表达基因数目分别为17 732、11 757和11 319 个。GO功能富集显示,差异基因主要富集于分子功能、催化活性等与细胞成长相关的GO功能。此外对KEGG通路进行了分析,结果显示差异基因显著富集于PI3K-Akt、细胞周期、癌症途径等与细胞分化增殖、凋亡相关的信号通路中,其中在幼体由浮游的大耳状幼体变态至附着的樽形幼体过程中,癌症途径的富集频率显著上升,表明其在幼体生长发育模式转换上发挥了关键作用。筛选的差异表达基因及预测的功能信息可为热带海参生长发育调控机制研究、人工繁育及分子改良应用提供参考。

     

    Abstract: Development of sea cucumber larvae needs to go through stages of auricularia, doliolaria, pentactula and juvenile. High mortality rate from metamorphosis and development of planktonic larvae to attachment stage of seedlings is a common problem in tropical sea cucumbers breeding. However, the gene regulation mechanism underlying the metamorphosis development of tropical sea cucumber is still unclear. In this study, we chose larval Holothuria leucospilota from four periods of early auricularia (A), mid auricularia (B), late auricularia (C) and doliolaria (D) as samples for high-throughput transcriptome sequencing to investigate the molecular mechanism underlying its metamorphosis development. The results show that a total of 83.6 GB Raw reads were generated, and 93 528 Unigenes were obtained by splicing. Pairwise comparisons between adjacent groups of the four sequencing libraries show that the number of genes with significant differential expression in A_vs_B, B_vs_C and C_vs_D were 17 732, 11 757 and 11 319, respectively. GO function enrichment shows that differential genes were mainly enriched in GO functions related to cell growth, such as molecular function and catalytic activity. In addition, KEGG pathways were analyzed, and the results show that the differential genes were significantly enriched in pathways related to cell differentiation, proliferation and apoptosis, such as PI3K-Akt, cell cycle and cancer pathways. Among them, the enrichment frequency of the pathway in cancer significantly increased during the metamorphosis process, which indicates a key role in the transformation of larval growth and development mode. The screened differentially expressed genes and predicted functional information can lay a foundation for the research on the regulatory mechanism of growth and development, artificial breeding and molecular improvement application of H. leucospilota.

     

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