Compared with ozone gas and ozone water, ozone ice not only maintains the original characteristics of ozone, but also shows higher ozone stability, better preservation effect and more convenient use. In this study, in order to explore methods for preparing high concentration ozone ice and improving ozone preservation rate, we used an ozone water integrated machine circulation device to prepare high-concentration ozone water. By adjusting the flow rate of ozone gas in ozone water integrated machine, the water temperature and pH in the circulating water tank, we determined the optimal conditions for preparing high-concentration ozone water. Then we used high concentration ozone water to prepare ozone ice, exploring methods for preparing high concentration ozone ice and improving ozone preservation rate by changing the preparation conditions and storage methods of ozone ice. The results show that ozone water of 50.27 mg·L−1
can be prepared in a short time by fixing the flow rate of ozone gas of 3 L·min−1
and adjusting the water temperature to 5 ℃ in circulating water tank and at pH of 3. Ozone ice with an ozone retention rate of 58.13% can be prepared by using liquid nitrogen to freeze high-concentration ozone water at temperature of 5 ℃ and pH of 5. In addition, the stability of ozone ice can be effectively improved by adding acetic acid as acidity regulator of ozone ice and storing the ozone ice at −40 ℃.