徐煜, 胡晓娟, 张淞, 徐武杰, 苏浩昌, 文国樑, 曹煜成. 5种因子对赤红球菌HDRR2Y去除氨氮和亚硝酸盐效应的影响[J]. 南方水产科学, 2023, 19(1): 67-74. DOI: 10.12131/20220044
引用本文: 徐煜, 胡晓娟, 张淞, 徐武杰, 苏浩昌, 文国樑, 曹煜成. 5种因子对赤红球菌HDRR2Y去除氨氮和亚硝酸盐效应的影响[J]. 南方水产科学, 2023, 19(1): 67-74. DOI: 10.12131/20220044
XU Yu, HU Xiaojuan, ZHANG Song, XU Wujie, SU Haochang, WEN Guoliang, CAO Yucheng. Effect of five factors on removing ammonia nitrogen and nitrite by Rhodococcus ruber HDRR2Y fermentation[J]. South China Fisheries Science, 2023, 19(1): 67-74. DOI: 10.12131/20220044
Citation: XU Yu, HU Xiaojuan, ZHANG Song, XU Wujie, SU Haochang, WEN Guoliang, CAO Yucheng. Effect of five factors on removing ammonia nitrogen and nitrite by Rhodococcus ruber HDRR2Y fermentation[J]. South China Fisheries Science, 2023, 19(1): 67-74. DOI: 10.12131/20220044

5种因子对赤红球菌HDRR2Y去除氨氮和亚硝酸盐效应的影响

Effect of five factors on removing ammonia nitrogen and nitrite by Rhodococcus ruber HDRR2Y fermentation

  • 摘要: 赤红球菌 (Rhodococcus ruber) 是常见的污水、废水处理微生物,为探究环境因子对赤红球菌HDRR2Y去除氨氮 (\rmNH_4^^ + -N)和亚硝酸盐 (\rmNO_2^^ - -N) 效应的影响,将HDRR2Y菌株发酵液添加至含有高浓度\rmNH_4^^ + -N或\rmNO_2^^ - -N的养殖水体中,通过国标法检测氨氮和亚硝酸盐浓度的变化,检验菌株培养液去除氨氮及亚硝酸盐的效果。按照温度、转速、盐度、接种菌量、底物浓度 (氨氮和亚硝酸盐) 5个因素进行Plackett-Burman实验设计,探讨这些因子对赤红球菌去除氨氮和亚硝酸盐效应的影响。结果显示,发酵过程中,菌株HDRR2Y浓度在36 h内从初始的5×104 CFU·mL−1增至4.08×109 CFU·mL−1。添加菌株培养液后,养殖水体氨氮质量浓度从初始的15 mg·L−1降至5.56 mg·L−1,去除率为62.96%;亚硝酸盐质量浓度从15 mg·L−1降至6.95 mg·L−1,去除率为59.37%。5种因子中,温度和氨氮浓度对菌株HDRR2Y去除氨氮影响最显著 (P<0.05),影响权重程度依次为:温度>氨氮浓度>转速>菌量>盐度;温度和转速是影响去除亚硝酸盐最显著的两个因子 (P<0.05),权重程度依次为:温度>转速>盐度>亚硝酸盐浓度>菌量。可见,菌株HDRR2Y培养液具有良好的去除氨氮、亚硝酸盐的作用,且温度是影响HDRR2Y去除氨氮、亚硝酸盐效率的最显著因子。

     

    Abstract: The bacteria Rhodococcus ruber is usually used in waste water and sewage treatment. In order to discuss the effect of environmental factors on R. ruber HDRR2Y's removing ammonia nitrogen and nitrite, we added the culture medium of R. ruber strain HDRR2Y to the aquaculture water containing high concentration of ammonia nitrogen (NH4 +-N) and nitrite (NO2 -N), and observed the changes of ammonia nitrogen and nitrite concentrations by the national standard method, so as to investigate the effect of the culture medium on the removal of ammonia nitrogen and nitrite. In addition, we carried out the Plackett-Burman experimental design according to five factors: temperature, rotating speed, salinity, inoculum amount, substrate (Ammonia nitrogen and nitrite) concentration, to explore the effect of these factors on the removal of ammonia nitrogen and nitrite by R. ruber. The results show that during the fermentation process, the mass concentration of strain HDRR2Y increased from 5×104 CFU·mL−1 to 4.08×109 CFU·mL−1 in the initial 36 h. After adding the culture medium, the mass concentration of ammonia nitrogen decreased from 15 mg·L−1 to 5.56 mg·L−1, with a removal rate of 62.96%, and that of nitrite decreased from 15 mg·L−1 to 6.95 mg·L−1, with a removal rate of 59.37%. Among the five factors, temperature and ammonia nitrogen concentration had the most significant effects on the removal of ammonia nitrogen by strain HDRR2Y (P<0.05) (Temperature>ammonia nitrogen concentration>rotation speed>biomass>salinity). Temperature and rotating speed were the two most significant factors affecting the removal of nitrite (P<0.05) (Temperature>rotating speed>salinity>nitrite concentration>bacterial count). It is showed that the culture medium of strain HDRR2Y is good for removing ammonia nitrogen and nitrite, and temperature is the most significant factor affecting the efficiency of HDRR2Y in removing ammonia nitrogen and nitrite.

     

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