Abstract: In order to explore the feasibility of removing and controlling the spread of antibiotic resistant genes (ARGs) by the fishing oxidant trichloroisocyanuric acid (C₃Cl₃N₃O₃) in the aquaculture water environment, we applied the real-time quantitative PCR to monitor and determine the ARGs concentrations in the offshore source water, storage water of sedimentation tank and reserved water after oxidation and disinfection for 29 d. The final concentration of trichloroisocyanuric acid used for oxidation and disinfection was 40 mg·L⁻¹. The target ARGs were commonly found in the aquaculture environments, including sul1, sul2, tetX, tetM, floR, cmlA and qnrA. The results show thatsul1, sul2, floR and tetX were the dominant ARGs among the above mentioned water samples. The number of types and total concentration of ARGs were the highest in offshore source water. The total concentrations of ARGs in the storage water of sedimentation tank were lower than those in the offshore source water, especially that the concentrations of sul2 andfloR were 0.86 and 0.34 lg lower than those in the offshore source water, respectively. After the oxidation and disinfection by trichloroisocyanuric acid, the total concentrations of ARGs in the reserved water decreased, and compared with the offshore source water, the concentrations of sul2 and floR decreased by 1.58 and 1.30 lg, respectively. The results indicate that offshore source water is the main source of ARGs in the aquaculture environment. Oxidation and disinfection treatment on offshore source water with trichloroisocyanuric acid can reduce the concentrations of common ARGs in the aquaculture water significantly, which is helpful to prevent and control the spread of ARGs in aquaculture environments.