Abstract: As important pathogenic factors of Aeromonas veronii, flagellin, quorum sensing and Type III secretion system (T3SS) are closely related to their pathogenicity and regulated by various environmental conditions. In order to study the effect of variation in external environmental factors on the pathogenic factors of A. veronii, we used the ascF, fliE and luxR factors of A. veronii as objects by real-time PCR method. The response of ascF, fliE and luxR genes to environmental factors such as temperature, pH, rotating speed and ions were explored at the transcription level. The results show that the three genes of A. veronii had a positive response to acidic environment (pH 6.5−7.0), low and medium speed (150−210 r·min⁻¹), Zn²⁺ and Mg²⁺. The response patterns of the three genes of the two strains of A. veronii with different molecular types were different, indicating that the pathogenic mechanisms of A. veronii might be different. Besides, the important virulence factors of A. veronii were regulated by environmental conditions and showed different regular change. The results provide references for further research on the pathogenesis of A. veronii.