李志成, 江飚, 钟志鸿, 李诗钰, 何润真, 唐嘉嘉, 李安兴. 硫酸铜治疗卵形鲳鲹淀粉卵涡鞭虫病的研究[J]. 南方水产科学, 2021, 17(3): 108-114. DOI: 10.12131/20200260
引用本文: 李志成, 江飚, 钟志鸿, 李诗钰, 何润真, 唐嘉嘉, 李安兴. 硫酸铜治疗卵形鲳鲹淀粉卵涡鞭虫病的研究[J]. 南方水产科学, 2021, 17(3): 108-114. DOI: 10.12131/20200260
LI Zhicheng, JIANG Biao, ZHONG Zhihong, LI Shiyu, HE Runzhen, TANG Jiajia, LI Anxing. Treatment of amyloodiniosis in golden pompano (Trachinotus ovatus) by copper sulfate[J]. South China Fisheries Science, 2021, 17(3): 108-114. DOI: 10.12131/20200260
Citation: LI Zhicheng, JIANG Biao, ZHONG Zhihong, LI Shiyu, HE Runzhen, TANG Jiajia, LI Anxing. Treatment of amyloodiniosis in golden pompano (Trachinotus ovatus) by copper sulfate[J]. South China Fisheries Science, 2021, 17(3): 108-114. DOI: 10.12131/20200260

硫酸铜治疗卵形鲳鲹淀粉卵涡鞭虫病的研究

Treatment of amyloodiniosis in golden pompano (Trachinotus ovatus) by copper sulfate

  • 摘要: 为更科学地使用硫酸铜治疗淀粉卵涡鞭虫病,该研究以卵形鲳鲹 (Trachinotus ovatus) 为动物模型,探讨了硫酸铜对眼点淀粉卵涡鞭虫 (Amyloodinium ocellatum) 生活史各个阶段的有效驱杀浓度和作用时间,并评估其对卵形鲳鲹幼鱼的安全质量浓度范围。结果显示,卵形鲳鲹幼鱼对硫酸铜的耐受性强,安全质量浓度小于43.06 mg·L−1。用3.13、0.78、0.20 mg·L−1硫酸铜溶液分别药浴处理10、30、60 min可100%驱杀涡孢子;用2.0、1.0、0.5 mg·L−1硫酸铜溶液分别药浴浸泡鱼体2、4、8 h可100%清除鱼体上的营养体;而包囊对硫酸铜的耐受性强,用100 mg·L−1硫酸铜溶液连续药浴,仍有90%以上的包囊能继续分裂。治疗实验显示,在0.2、0.4 mg·L−1硫酸铜溶液中连续药浴10 d,对患病鱼的相对保护率分别为80%和90%,表明使用低浓度硫酸铜溶液连续药浴可有效治疗卵形鲳鲹淀粉卵涡鞭虫病。

     

    Abstract: In order to use copper sulfate scientifically to treat the disease caused by parasite Amyloodinium ocellatum in farmed fish, golden pompano (Trachinotus ovatus) was used as an animal model to study the copper sulfate's effective concentration and time of killing of each stage of A. ocellatum. The safety concentration of copper sulfate to juvenile golden pompano was evaluated. Results show that the juveniles had good tolerance to copper sulfate and the safety concentration was less than 43.06 mg·L−1. The effective concentrations of copper sulfate to kill full dinospores within 10, 30 and 60 min were 3.13, 0.78 and 0.20 mg·L−1, respectively. At copper sulfate concentrations of 2, 1 and 0.5 mg·L−1, trophonts were detached from the host within 2, 4 and 8 h, respectively. However, tomonts were highly resistant to copper sulfate because they divided when being incubated with 100 mg·L−1 copper sulfate solution. Additionally, the relative protection rates of pompanos infected with A. ocellatum were 80% and 90% when being immersed continuously in 0.2 and 0.4 mg·L−1 copper sulfate solutions respectively for 10 d. These results imply that the immersion to copper sulfate solution can protect golden pompano from A. ocellatum infection at low concentration.

     

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