陈旭, 左涛, 周胜杰, 杨蕊, 于刚, 秦传新, 马振华. 尖吻鲈幼鱼运输密度和时长对运输水质及其复苏率和抗氧化能力的影响[J]. 南方水产科学, 2021, 17(2): 122-128. DOI: 10.12131/20200169
引用本文: 陈旭, 左涛, 周胜杰, 杨蕊, 于刚, 秦传新, 马振华. 尖吻鲈幼鱼运输密度和时长对运输水质及其复苏率和抗氧化能力的影响[J]. 南方水产科学, 2021, 17(2): 122-128. DOI: 10.12131/20200169
CHEN Xu, ZUO Tao, ZHOU Shengjie, YANG Rui, YU Gang, QIN Chuanxin, MA Zhenhua. Effects of transport density and duration on transport water quality, resuscitation rate and antioxidant ability in juvenile Lates calcarifer[J]. South China Fisheries Science, 2021, 17(2): 122-128. DOI: 10.12131/20200169
Citation: CHEN Xu, ZUO Tao, ZHOU Shengjie, YANG Rui, YU Gang, QIN Chuanxin, MA Zhenhua. Effects of transport density and duration on transport water quality, resuscitation rate and antioxidant ability in juvenile Lates calcarifer[J]. South China Fisheries Science, 2021, 17(2): 122-128. DOI: 10.12131/20200169

尖吻鲈幼鱼运输密度和时长对运输水质及其复苏率和抗氧化能力的影响

Effects of transport density and duration on transport water quality, resuscitation rate and antioxidant ability in juvenile Lates calcarifer

  • 摘要: 该试验采用塑料袋密闭充氧运输方式,探讨3种运输密度 (D1=14 kg·m−3、D2=21 kg·m−3、D3=28 kg·m−3) 下模拟运输24 h和运输密度7 kg·m−3下模拟6个不同运输时长 (T1=4 h、T2=8 h、T3=12 h、T4=16 h、T5=20 h、T6=24 h) 对运输水质、尖吻鲈 (Lates calcarifer) 幼鱼复苏率和抗氧化能力的影响。结果显示,随着运输密度和时间的增加,运输水体中氨氮 (NH4 +-N)和亚硝态氮 (NO2 -N) 升高而溶解氧 (DO)、酸碱度 (pH)和复苏率降低;幼鱼成活率均为100%。运输密度结果显示,D1和D2组总超氧化物歧化酶 (T-SOD) 活性显著低于对照组 (P<0.05);D3组过氧化物酶 (POD) 活性均显著低于D1、D2和对照组 (P<0.05);D2与D3组相比丙二醛 (MDA) 含量差异不显著,均显著高于D1和对照组 (P>0.05)。运输时长结果显示,T3与T4组相比T-SOD活性差异不显著,显著高于T1、T2、T5和T6组 (P<0.05);各试验组POD活性均显著高于对照组 (P<0.05);T2组MDA含量与T4组相比差异不显著,显著高于T1、T3、T5、T6和对照组 (P<0.05)。研究表明,随着运输密度和时间的增加,尖吻鲈幼鱼运输水质恶化,复苏率降低,对抗氧化能力影响显著。综合分析,在水温24 ℃、盐度32、pH 7.92的条件下用塑料袋密封充氧运输的体长为 (1.42±0.19) cm的尖吻鲈幼鱼24 h时,密度不超过21 kg·m−3为宜。

     

    Abstract: The experiment was conducted to investigate the effects of simulated transportation for 24 h with three transport densities (D1=14 kg·m−3, D2=21 kg·m−3, D3=28 kg·m−3) and six different transport durations (T1=4 h, T2=8 h, T3=12 h, T4=16 h, T5=20 h, T6=24 h) at 7 kg·m−3 on the transport water quality, resuscitation rate and antioxidant capacity of juvenile Lates calcarifer. The results show that with the increase of transport density and duration, the contents of NH4 +-N and NO2 -N in the transport water increased, while the DO content, pH and resuscitation rate decreased but the survival rate was 100%. The results of transport density test show that the T-SOD activities in D1 and D2 groups were significantly lower than that in the control group (P<0.05); the POD activity in D3 group were significantly lower than those in D1 group, D2 group and the control group (P<0.05); the MDA content in D2 group was not significantly different from that in D3 group, which was significantly higher than those in D1 group and control group (P>0.05). The results of transport time test show that there was no significant difference in T-SOD activity between T3 and T4 groups, which were significantly higher than those in T1, T2, T5 and T6 groups (P<0.05); the POD activity in each experimental group was significantly higher than that in the control group (P<0.05); the MDA content in T2 group was not significantly different from that in T4 group, but was significantly higher than those in T1, T3, T5, T6 groups and control group (P<0.05). It is shown that with the increase of transport density and duration, the water has deteriorated and the resuscitation rate decreased, having significant effect on the antioxidant ability of L. calcarifer juveniles. In conclusion, under conditions of water temperature 24 ℃, salinity 32 and pH 7.92, with body length of (1.42 ± 0.19) cm, the density should not exceed 21 kg·m−3 for 24 h.

     

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