花鲈垂体和下丘脑中生物钟基因在3种光周期下的表达节律分析

Circadian rhythmicity of clock genes in pituitary and hypothalamus of spotted sea perch (Lateolabrax maculates) under three photoperiod conditions

    摘要
  • 摘要: 花鲈 (Lateolabrax maculatus) 是中国重要的水产养殖鱼类，其繁殖活动受光周期调控。文章研究了3种光周期 光 (L) 暗 (D) 比分别为16L∶8D、12L∶12D和8L∶16D 条件下，7个重要生物钟基因 (Bmal2、Npas4、Per2、Cry1、Cry1a、Cry2 及Timeless) 在花鲈垂体和下丘脑中的昼夜表达规律。结果表明，在12L∶12D条件下垂体中Per2、Cry1、Cry2、Cry1a、Timeless表现出昼夜节律性，下丘脑中Per2、Cry2、Cry1、Timeless表现出昼夜节律性，相同基因在垂体和下丘脑两种组织中的昼夜节律不同，长光照或短光照会改变昼夜节律的震荡强弱，也会改变峰值相位，部分基因在长光照或短光照下会出现失去昼夜节律性的现象。

     

    Abstract: Lateolabrax maculatus is an important economic fish in China. Its reproduction is regulated by photoperiod. In this study, seven important clock genes (Bmal2, Npas4, Per2, Cry1, Cry1a, Cry2 and Timeless) were detected in the pituitary and hypothalamus of L. maculatus under three photoperiod conditions (16L∶8D, 8L∶16D and 12L∶12D). The results show that under 12L∶12D condition, Per2, Cry1, Cry2, Cry1a and Timeless displayed daily rhythmic expression in pituitary, and Per2, Cry2, Cry1 and Timeless displayed daily rhythmic expression in hypothalamus. The circadian rhythms of the same gene in pituitary and hypothalamus were also different. Long day (16L∶8D) or short day (8L∶16D) could change the amplitude of circadian rhythm and the peak phase, and some genes lost circadian rhythms under this condition.

     

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