Abstract: In this study, we compared the feeding method and feeding time of 17β-estradiol to explore the feminization method of XY Pelteobagrus fulvidraco, and then established three families (XY♀×XY♂) to compare the survival rate and sex reversal rate with sex molecular. The results show that the females in Artemia sp. group could not be induced to develop well from the 5^th day of opening, but the induced reversal rate was 75.20% on the 1^st day of opening, the survival rate induced  from the 1^st day of opening was not significantly different from that of Artemia sp. group, while the sex reversal rate was significantly higher than that of Artemia group; the combination of molecular markers Y1 and Y2 could identify the genetic sex of P. fulvidraco accurately; there was no significant difference in the survival rate and sex ratio of each genotype among the three families in the control group, and the survival rate was 51.00%−56.67%. The proportion of XX female and XY male individuals were 90.91%−97.44% and 94.56%−95.96%, respectively. There was no significant difference in the survival rate and sex reversal rate among the three families in the induction group. The result shows that using 50 mg·kg⁻¹ E₂ mixed with micro pellet feed to induce 30 d from the 1^st day of opening can screen out well-developed female XY/YY P. fulvidraco effectively by combination of genetic and phenotypic sex identification.