复合酶法提取石花菜粗多糖工艺的响应面优化

Optimization of multi-enzymatic extraction of polysaccharide from Gelidium amansii by response surface methodology

摘要: 为优化复合酶法m (木瓜蛋白酶)∶m (纤维素酶) =2∶1提取石花菜(Gelidium amansii)粗多糖的工艺条件，在单因素实验基础上进行三因素三水平的Box-Benhnken中心组合实验，并根据回归分析确定石花菜粗多糖的复合酶法最佳提取工艺为复合酶添加量2.30%、酶解温度60.43 ℃、料液比1∶32 (g·mL⁻¹)。在此条件下酶解120 min，石花菜粗多糖的提取率可达15.98%。

Abstract: To optimize the multi-enzymatic extraction process of polysaccharides from Gelidium amansii, we designed a three-factor and three-level Box-Benhnken center-united experiment based on single factor test, and conducted a regression analysis. The optimum technological parameters of enzymatic extraction of G. amansii polysaccharide were: enzymes addition 2.30%, enzymatic hydrolysis temperature 60.43 ℃, ratio of material to water 1∶32 (g·mL⁻¹). The extraction yield of polysaccharide was up to 15.98% after 120 min of enzymatic hydrolysis under these conditions.