Abstract: We obtained the full-length cDNA sequence of GLUT1 from Penaeus monodon by rapid amplification of cDNA ends (RACE), and investigated the expression of GLUT1 at different larval developmental stages, in different tissues, and under low salinity stress by quantitative real-time PCR. The total cDNA sequence of PmGLUT1 open reading frame (ORF) was 1 476 bp, encoding 491 amino acids. From zygote to postlarva stages, the expression of PmGLUT1 fluctuated but showed an increasing trend. The PmGLUT1 was expressed in all tested tissues with the highest expression in gill tissue, followed by hepatopancreas, and the lowest expression was in ovary. After acute low salinity stress, the expression level of PmGLUT1 mRNA in hepatopancreas was significantly higher than that in the control group, while the expression level in gill was significantly lower than that in the control group (P<0.05). The results show that PmGLUT1 might play an important role at different larval developmental stages and under low salinity stress, which provides a theoretical basis for studying the molecular mechanism of PmGLUT1 in larval development and salinity adaptation of P. monodon.