Abstract: Fish anesthetic eugenol, which has good antibacterial and bactericidal effects, has presented great efficacy on the food borne pathogens as well as the pathogenic bacteria in aquaculture. In order to investigate the antibacterial effect of eugenol on the typical pathogenic bacteria in aquaculture, we studied the antibacterial effect of eugenol on Vibrio anguillarum. After 24-hour incubation, the diameter of the inhibition zone was (21.13±0.74) mm at 6 400 μg·mL^–1 of eugenol, indicating that V. anguillarum was extremely sensitive to eugenol. It is shown that V. anguillarum did not grow while the eugenol concentration was over 400 μg·mL^–1. Hence, the minimum inhibitory concentration (MIC) was 400 μg·mL^–1 of eugenol against the bacteria. The solution in the cells of 400 μg·mL^–1, 800 μg·mL^–1 and 1 600 μg·mL^–1 were continually incubated for 24 h after being scribbled on the plate surface. When the concentration of eugenol were over 800 μg·mL^–1, no V. anguillarum grew in the plates. It is indicated that the minimum bactericidal concentration (MBC) of eugenol against V. anguillarum was 800 μg·mL^–1, and eugenol has excellent antibacterial effects on V. anguillarum.