Abstract: We constructed the sRNA srvg17985 knock-out mutant in Vibrio alginolyticus ZJ-T by using homologous recombination, so as to compare the biological characteristics of growth in LBS medium, motility, extracellular protease secretion, iron utilization, antibiotic resistance and metabolism between the wild type and the mutant strains. The results show that the absence of sRNA srvg17985 did not affect the growth in LBS medium, motility, extracellular protease secretion, iron utilization, antibiotic resistance and most of the tested carbon and nitrogen sources metabolism in V. alginolyticus. However, the mutant showed better utilization of laminarin, pectin and dihydroxyaceton, and gained the ability to utilize alanine-aspartic acid (Ala-Asp) as the sole nitrogen source.