Abstract: We investigated short-term fin tissues cultivation of Pseudorasbora parva by explant technique, and studied its Ag-NORs, and C-banding patterns at the same time. Results are as follows: 1) Cells from the fin tissues of P.parva migrate out around tissues at 6thday through MEM medium, the most suitable one, and are fibroblast-like during cultivation; 2) A pair of Ag-NORs is found on the end of no.23 chromosome short arm, and the NORs are polymorphic, while no Ag-NORs combination has happened in this fish. 3) C-bands are located mainly in the terminal and centromere regions of chromosomes, and a few in the interstitial regions. In particular, most of the short arm on chromosome No.23 shows strong positive C-bands, which is corresponded with Ag-NORs region.