3个鲤群体的微卫星标记与生长性状相关性分析

Correlation analysis of microsatellite DNA markers with growth trait among 3 breeding populations of common carp

  • 摘要: 利用筛选出鲤(Cyprinus carpio)的15对多态性较高的微卫星引物,分析其与黄河鲤(C.carpio haematopterus Temminck et Schlegel)、建鲤(C.carpio var.jian)和黑龙江野鲤(C.carpio haematopterus)群体中90个个体的体质量、体长、体厚和体高性状的相关性。利用SAS的一般线性模型(GLM)对15个微卫星标记与3个鲤群体的生长性状进行显著性检验。结果表明, Mfw5与黄河鲤的体高相关(P < 0.05);Hlj013、Cca09和Mfw7均与建鲤的体质量、体长和体高相关(P < 0.05);Mfw2与建鲤的体质量和体厚相关(P < 0.05);Mfw29与建鲤的体质量相关(P < 0.05);Mfw6与黑龙江野鲤的体质量、体长、体厚和体高均相关(P < 0.05);Mfw4与黑龙江野鲤的体质量、体长和体高相关(P < 0.05);Mfw11与黑龙江野鲤的体厚和体高相关(P < 0.05)。对同一性状不同基因型间进行多重比较,找到3个群体中与生长性状相关的基因型。

     

    Abstract: We screened out the molecular makers of 15 microsatellite loci for common carp(Cyprinus carpio), and compared these markers with Huanghe carp (C.carpio haematopterusTemminck et Schlegel), Jian carp (C.carpio var. jian) and Heilongjiang carp (C.carpio haematopterus) populations in aspects of body weight, body length, body width and body depth. The results based on GLM show that Mfw5 is significantly correlated with body depth for Huanghe carp (P < 0.05). For Jian carp population, Hlj013, Cca09 and Mfw7 are significantly correlated with body weight, body length and body depth (P < 0.05); Mfw2 is significantly correlated with body weight and body width (P < 0.05); while Mfw29 is significantly correlated with body weight (P < 0.05). For Heilongjiang carp population, Mfw6 is significantly correlated with body weight, body length, body width and body depth (P < 0.05); Mfw4 is significantly correlated with body weight, body length and body depth (P < 0.05); Mfw11 is significantly correlated with body width and body depth (P < 0.05). Furthermore, the genes and genotypes related to growth traits in the 3 carp populations are found.

     

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