Abstract: Four nitrofuran metabolites, including semicarbazide (SEM), 1-amino-hydantoin (AHD), 3-amino-2-oxazolidinone (AOZ) and 5-methylmorpholino-3-amino-2-oxazolidinone (AMOZ) in Litopenaeus vannamei, were determined by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). SEM-¹³C-¹⁵N₂, AHD-¹³C₃, AOZ-D₄ and AMOZ-D₅ were selected as internal standard substances; the samples were derived by 2-nitrobenzaldehyde, extracted with ethyl acetate and cleaned up with n-hexane. Four targeted compounds were completely separated within 8 minutes by gradient elution of methanol and 0.5 mmol·L^-1 ammonium acetate including 0.1% fomic acid, and they have good linear relation within 0.4~20.0 ng·mL^-1(R > 0.999), the limit of detection being 0.03~0.15 μg·kg^-1. The recovery is 92.0%~107.3% (n=6) and the relative standard deviation is 3.1%~8.7% at 0.5 μg·kg^-1, 1.0 μg·kg^-1 and 2.0 μg·kg^-1.The method achieved satisfactory result in the FAPAS (Food Analysis Performance. Assessment Scheme) International Proficiency Testing.