Abstract: A 56-day feeding trial on pompano (Trachinotus ovatus) was carried out to study the effects of dietary inositol on its growth, feed utilization and blood biochemical index. Diets with 5 levels of inositol (350 mg · kg^-1, 458 mg · kg^-1, 507 mg · kg^-1, 720 mg · kg^-1 and 1 050 mg · kg^-1) were fed to pompano at initial weight of (8.30 ±0.11) g, respectively. Weight gain (WG) and special growth rate (SGR) of 720 mg · kg^-1 group are significantly higher (P < 0.05) than those of the other groups. Viscerasomatic index (VSI) is not significantly (P > 0.05) affected by inositol. Survival of 458 mg · kg^-1, 507 mg · kg^-1 and 720 mg · kg^-1 groups are significantly higher than that of 350 mg · kg^-1 and 1 050 mg · kg^-1 groups(P < 0.05). The moisture and lipid content of 507 mg · kg^-1 group are significantly higher and lower than those of the other groups except the 720 mg · kg^-1 one (P < 0.05), respectively.No significant difference is found in crude protein content (P > 0.05).The highest glucose (GLU) content is found in 720 mg · kg^-1 group, which is significantly higher than that of 350 mg · kg^-1 and 458 mg · kg^-1 groups (P < 0.05).Low density lipoprotein cholesterol (LDCL) of 507 mg · kg^-1 group is significantly lower than that of the other groups except 1 050 mg · kg^-1 one(P < 0.05).Supplementation of dietary inositol can significantly decrease triacylglycerol (TG) content, but has no effect on total protein (TP), blood urea nitrogen (BUN), total cholesterol (TC) and high density lipoprotein cholesterol (HDLC).