Abstract: The growth, feed utilization and whole-body composition of six Penaeus monodon families (hsy5-5, hsy7-7, hsy8-5, hsy10-10, hsy12-13 and hsy15-15) were studied. These families were established using wild broodstock from Sanya Bay by the method of artificial insemination. In the feed utilization and growth experiment, the weight gain and feed conversion ratio (FCR) of hsy10-10 were superior to the others. The survival rates of hsy8-5, hsy10-10 and hsy12-13 were higher than the others but not significant (P>0.05).When cultured in concrete tank in mix, the survival rate of hsy10-10 was as high as 90 percent, higher than other five families (hsy8-5, hsy12-13, hsy7-7, hsy15-15 and hsy5-5) by 13.3%, 13.3%, 20.0%, 23.3% and 30.0%, respectively. The total month weight gain of hsy10-10 and hsy8-5 was higher than other four families. When cultured in earthen pond, the final body weight of hsy10-10 was higher than the average of the six families by 13.1%. It was concluded that the survival rate, weight gain and FCR of family hsy10-10 were superior to the other families, and the whole shrimp of hsy10-10 had the highest contents of crude protein and lipid and the lowest of moisture among six families.