牡蛎及其酶解产物抗皮肤光老化的初步研究

吕佳桐; 林海生; 秦小明; 章超桦; 曹文红; 高加龙; 郑慧娜

doi:10.12131/20200141

牡蛎及其酶解产物抗皮肤光老化的初步研究

doi: 10.12131/20200141

吕佳桐^1,,
林海生^{1, 2},
秦小明^{1, 2, ,},
章超桦^{1, 2},
曹文红^{1, 2},
高加龙^{1, 2},
郑慧娜^{1, 2}

1.
广东海洋大学食品科技学院，广东湛江 524088
2.
广东省水产品加工与安全重点实验室/广东普通高等学校水产品深加工重点实验室/国家贝类加工技术研发分中心 (湛江)/南海生物资源开发与利用协同创新中心，广东湛江 524088

基金项目: 现代农业产业技术体系建设专项资金 (CARS-49)；国家重点研发计划项目“水产品非宏量营养素的健康功效解析” (2018YFD0901105)

详细信息

作者简介:
吕佳桐 (1995—)，女，硕士研究生，研究方向为海洋功能活性物质。E-mail: 1141432665@qq.com

通讯作者:
秦小明 (1964—)，男，博士，教授，从事水产品精深加工、贝类活性物质研究。E-mail: xiaoming0502@21cn.scom

中图分类号: TS 254.9
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- 被引次数: 0
出版历程
- 收稿日期: 2020-07-07
- 修回日期: 2020-08-21
- 网络出版日期: 2020-12-05
- 刊出日期: 2021-02-04

Preliminary study on resistance of oyster and its enzymatic hydrolysis products to skin photoaging

Jiatong LÜ^1
,,
Haisheng LIN^{1, 2},
Xiaoming QIN^{1, 2
, ,},
Chaohua ZHANG^{1, 2},
Wenhong CAO^{1, 2},
Jialong GAO^{1, 2},
Huina ZHENG^{1, 2}

1.
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
2.
Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety/Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution/National Research and Development Branch Center for Shellfish Processing (Zhanjiang)/South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhanjiang 524088, China

摘要

摘要: 文章探讨了香港牡蛎 (Crassostrea hongkongensis) 肉粉 (Oyster meat, OM) 及其酶解产物 (Oyster hydrolysis, OH) 对紫外线诱导的小鼠 (Mus musculus) 皮肤光老化的保护作用。紫外线 (UVA+UVB) 每天照射构建小鼠皮肤光老化模型并灌胃OM和OH，分为低、中、高三组 (30、90、180 mg·kg⁻¹) 给药，持续8周。结果表明，OM和OH可减轻小鼠皮肤皱纹，提高皮肤弹性，减少皮下血管的萎缩，防止血管通透性增加；皮肤HE染色结果表明，中高剂量的OM和OH可显著减缓表皮层过度角化增厚 (P<0.05)；Masson染色和醛品红染色结果表明，OM和OH可减少胶原纤维的卷曲和降解，避免弹性纤维的异常增生，恢复真皮细胞外基质网络的排列；与模型组相比，OM和OH可使皮肤中超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化氢酶 (GSH-Px) 的活力显著提高，同时使丙二醛 (MDA) 和8-羟基脱氧鸟苷 (8-OHDG) 的浓度降低 (P<0.05)；OM和OH可抑制基质金属蛋白酶 (MMP-3、MMP-9) 的表达，显著降低真皮中羟脯胺酸 (Hyp) 的降解；此外，高剂量的OH可显著降低皮肤中炎症因子细胞白介素6 (IL-6) 和肿瘤坏死因子 (TNF-α) 的表达，并通过提高转化生长因子 (TGF-β) 来提高皮肤抗炎能力。
- 牡蛎 /
- 酶解产物 /
- 皮肤 /
- 光老化 /
- 抗氧化 /
- 紫外线辐射
Abstract: To explore the protective effects of Hong Kong oyster (Crassostrea hongkongensis) meat (OM) and its enzymatic hydrolysis (OH) products on UV-induced photoaging of mouse (Mus musculus) skin. Ultraviolet rays (UVA+UVB) were irradiated every day to build a mouse skin photoaging model. The mice were given OM and OH daily and divided into low, medium and high dose groups (30, 90, 180 mg·kg⁻¹) for eight weeks. The results show that OM and OH can reduce mice skin wrinkles, improve skin elasticity, reduce subcutaneous blood vessel atrophy, and prevent increase in vascular permeability. The results of skin HE staining show that medium and high doses of OM and OH can slow down hyperkeratosis and epidermis thickening significantly (P<0.05). Masson staining and aldehyde fuchsin staining results show that OM and OH can reduce the curling and degradation of collagen fibers, avoid abnormal proliferation of elastic fibers, and restore the arrangement of the dermal extracellular matrix network. Compared with the model group, OM and OH can increase the enzyme activity of superoxide dismutase (SOD) and glutathione catalase (GSH-Px) in skin, while malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHDG) concentration decreased significantly (P<0.05). OM and OH can inhibit the expression of matrix metalloproteinases (MMP-3, MMP-9), and reduce the degradation of hydroxyproline (Hyp) in the dermis significantly. In addition, high doses of OH can reduce the expression of inflammatory factors, interleukin 6 (IL-6) and tumor necrosis factor (TNF-α) in the skin significantly, and increase the skin's anti-inflammatory ability by increasing transforming growth factor (TGF-β).
- Oyster /
- Enzymatic hydrolysis product /
- Skin /
- Photoaging /
- Antioxidant /
- Ultraviolet radiation

HTML全文

图 1 牡蛎及其酶解产物对小鼠光老化皮肤表征的影响

a. 小鼠背部皮肤的宏观外观；b. 小鼠皮下血管状态；图1-b中黑色箭头指示血管通透性增加引发炎症，黄色圆圈区域指示血管萎缩

Figure 1. Effects of oyster and its enzymatic hydrolysis products on skin characterization of photoaging skin in mice

a. Macroscopic appearance of skin on the back of mice; b. State of subcutaneous blood vessels in mice; the black arrows in Figure 1-b indicate inflammation caused by increased vascular permeability, and the yellow circle area indicates vascular atrophy.

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图 2 牡蛎及其酶解产物对小鼠光老化皮肤结构和表皮层厚度的影响

同一指标中标有不同字母代表差异显著 (P<0.05)；图4~图6同此

Figure 2. Effect of oysters and their enzymatic hydrolysis products on the structure and thickness of photoaging skin in mice

For the same indicator, different letters indicate significant difference (P<0.05). The same case in Figure 4−Figure 6.

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图 3 牡蛎及其酶解产物对小鼠光老化皮肤中胶原纤维 (a) 和弹力纤维 (b) 的影响 (100×)

图3-a中黑色箭头指示混乱卷曲的胶原纤维，黄色箭头指示排列规律的胶原纤维；图3-b中黑色箭头指示弹力纤维在表皮层的异常增生

Figure 3. Effect of oysters and their enzymatic hydrolysis products on collagen fibers (a) and elastic fibers (b) in photoaging skin of mice (100×)

In Figure 3-a, the black arrows indicate the disorderly and curly collagen fibers, and the yellow arrows indicate the regularly arranged collagen fibers; in Figure 3-b, the black arrows indicate the abnormal proliferation of elastic fibers in the epidermis.

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图 4 牡蛎及其酶解产物对超氧化物歧化酶、谷胱甘肽过氧化氢酶、丙二醛和8-羟基脱氧鸟苷的影响

Figure 4. Effect of oysters and their enzymatic hydrolysis products on SOD, GSH-Px, MDA and 8-OHDG

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图 5 牡蛎及其酶解产物对MMP-3 (a)、MMP-9 (b)和羟脯胺酸 (c) 的影响

Figure 5. Effect of oysters and their enzymatic hydrolysis products on MMP-3 (a), MMP-9 (b) and Hyp (c)

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图 6 牡蛎及其酶解产物对白介素6、肿瘤坏死因子α (b) 和转化生长因子β (c) 的影响

Figure 6. Effect of oysters and their enzymatic hydrolysis products on IL-6 (a), TNF-α (b) and TGF-β (c)

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表 1 分组、照射及灌胃剂量

Table 1. Grouping, irradiation and intragastric dosage

组别　　　　 Group	是否照射UVA+UVB Whether to irradiate UVA+UVB	每日灌胃剂量 Daily intragastric dose/(mg·kg⁻¹)
空白组 Blank group (B)	否	−
模型组 Model group (M)	是	−
阳性对照组 Positive control group (P)	是	10
全牡蛎低剂量组 Oyster meat low dose (OM-L)	是	30
全牡蛎中剂量组 Oyster meat medium dose (OM-M)	是	90
全牡蛎高剂量组 Oyster meat high dose (OM-H)	是	180
牡蛎酶解低剂量组 Oyster hydrolysis low dose (OH-L)	是	30
牡蛎酶解中剂量组 Oyster hydrolysis medium dose (OH-M)	是	90
牡蛎酶解高剂量组 Oyster hydrolysis high dose (OH-H)	是	180
注：每日光照后给药，维A酸的剂量以维A酸质量百分比计，全牡蛎粉及牡蛎酶解粉的剂量以蛋白质量百分比计 Note: After daily light administration, the dosage of retinoic acid is based on the quality of retinoic acid, and the dosages of whole oyster powder and oyster enzymolysis powder are based on protein quality.

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表 2 皮肤皱纹等级评分标准

Table 2. Skin wrinkle rating scale

评分 Score	评分标准 Grading
0	无褶皱或松弛，无增厚，纵向细条纹贯穿背部，随运动出现或消失
1	皮肤大部分为纵向细纹，少部分横向细纹，可随运动出现或消失
2	尚存在少量纵向细纹，大部分横向细纹，随运动出现或消失，皮肤稍有增厚
3	纵向细纹全部消失，出现少量横向浅皱纹，不随运动出现或消失，皮肤稍有增厚
4	持续存在横向深皱纹和松弛，皮肤泛红，皮肤明显增厚
5	持续存在大量横向粗糙深皱纹和松弛，出现小红斑，皮肤明显增厚
6	严重的皱纹，大面积红斑，肿瘤或病变的发展，皮肤增生异常

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表 3 皮肤表征评价

Table 3. Evaluation of skin characterization $ \overline { X}{\bf \pm {SD}}$

组别　　　 Group	皮肤评分 Skin score	提皮恢复时间 Skin recovery time/s	皮肤总厚度 Total skin thickness/mm
空白组 Blank group (B)	0.00±0.00^f	1.97±0.19^e	0.39±0.03^f
模型组 Model group (M)	5.60±0.49^a	8.40±1.29^a	1.01±0.06^a
阳性对照组 Positive control group (P)	2.00±0.63^de	2.54±1.00^de	0.66±0.05^e
全牡蛎低剂量组 Oyster meat low dose (OM-L)	3.80±0.40^b	4.99±0.68^b	0.92±0.07^b
全牡蛎中剂量组 Oyster meat medium dose (OM-M)	3.20±0.40^bc	4.31±0.27^bc	0.83±0.05^cd
全牡蛎高剂量组 Oyster meat high dose (OM-H)	2.80±0.40^c	2.30±0.38^e	0.71±0.04^e
牡蛎酶解低剂量组 Oyster hydrolysis low dose (OH-L)	3.20±0.40^bc	4.80±0.20^b	0.89±0.07^bc
牡蛎酶解中剂量组 Oyster hydrolysis medium dose (OH-M)	2.60±0.49^cd	3.43±0.25^cd	0.78±0.06^d
牡蛎酶解高剂量组 Oyster hydrolysis high dose (OH-H)	1.40±0.49^e	2.21±0.42^e	0.65±0.02^e
注：每列不同字母代表差异显著 (P<0.05) Note: Different letters in the same column represent significant difference (P<0.05).

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