三唑磷对翡翠贻贝神经毒性与氧化损伤效应的性别差异研究

张林宝; 陈海刚; 田斐; 孙伟; 张喆; 蔡文贵

doi:10.12131/20200097

三唑磷对翡翠贻贝神经毒性与氧化损伤效应的性别差异研究

doi: 10.12131/20200097

中国水产科学研究院南海水产研究所/农业农村部南海渔业资源环境科学观测实验站/广东省渔业生态环境重点实验室，广东广州 510300

基金项目: 广东省自然科学基金项目 (2018A030313583)；中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助项目 (2017YB09)；中国水产科学研究院基本科研业务费专项 (2017HY-ZD0204)；广东省科技计划项目 (2019B121201001)

详细信息

作者简介:
张林宝 (1984—)，女，博士，副研究员，从事海洋环境毒理学研究。E-mail: zhanglinbao1984@163.com

中图分类号: X 55，S 949
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出版历程
- 收稿日期: 2020-05-08
- 修回日期: 2020-07-24
- 网络出版日期: 2020-11-18
- 刊出日期: 2020-12-04

Gender differences in neurotoxicity and oxidative damage of triazophos on Perna viridis

South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of South China Sea Fishery Resources and Environment, Ministry of Agriculture and Rural Affairs/Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China

摘要

摘要: 文章以具有环境指示功能的翡翠贻贝 (Perna viridis) 为研究对象，首次研究了三唑磷对其神经毒性和氧化损伤效应的性别差异性。实验测定了35 μg·L⁻¹[1/100 96 h 半致死浓度 (LC₅₀)]三唑磷慢性暴露15 d后雌、雄翡翠贻贝鳃组织中乙酰胆碱酯酶 (AchE) 和几种抗氧化指标的变化特征。结果显示，三唑磷作用15 d后雌性翡翠贻贝鳃组织中AchE活力无明显变化，而雄性个体中AchE活力被显著抑制。几种抗氧化指标对三唑磷暴露也表现出一定的性别差异，其中超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 活力在雌、雄翡翠贻贝鳃组织中均被显著抑制，丙二醛 (MDA) 均显著升高。谷胱甘肽硫转移酶 (GST) 活力和还原型谷胱甘肽 (GSH) 含量变化则表现出明显的性别差异，仅雄性翡翠贻贝GST活力和GSH含量有显著性变化。综上，三唑磷农药对翡翠贻贝鳃组织表现出明显的神经毒性和氧化损伤效应，其中雄性翡翠贻贝对三唑磷暴露的响应更为敏感。
- 翡翠贻贝 /
- 三唑磷 /
- 神经毒性 /
- 氧化损伤 /
- 性别差异
Abstract: We studied the gender differences of the neurotoxicity and oxidative damage effects of triazophos on Perna viridis that has function of environmental indicator for the first time. The changes of acetylcholinesterase (AchE) and several antioxidant indices in gill tissues of male and female were determined after 15 d of chronic exposure to 35 μg·L⁻¹ (1/100 96 h LC₅₀). The results show that there was no significant change in the AchE activity in female's gill tissue after being treated with triazophos for 15 d, while the AchE activity in male was significantly inhibited. The activities of superoxide dismutase (SOD) and catalase (CAT) in gill tissues of both male and female were significantly inhibited, and the malondialdehyde (MDA) content was significantly increased. The activity of glutathione S-transferase (GST) and the content of reduced glutathione (GSH) showed significant gender differences. Only the GST activity and GSH content in male had significant changes. In conclusion, triazophos shows obvious neurotoxicity and oxidative damage effects on gill tissue of P. viridis, and the male was more sensitive to triazophos exposure.
- Perna viridis /
- Triazophos /
- Neurotoxic effect /
- Oxidative stress /
- Gender difference

HTML全文

图 1 三唑磷对雌、雄翡翠贻贝鳃组织中乙酰胆碱酯酶、谷胱甘肽硫转移酶、超氧化物歧化酶、过氧化氢酶活力以及还原型谷胱甘肽、丙二醛含量的影响

*. P<0.05

Figure 1. Effects of triazophos exposure on activities of AchE, GST, SOD and CAT, and contents of GSH and MDA in gills of female and male P. viridis

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参考文献(41)

[1]	钟志, 刘琴, 刘士忠, 等. 三唑磷对浙江沿岸海域水生生物的影响[J]. 广州化工, 2010, 38(5): 215-216. doi: 10.3969/j.issn.1001-9677.2010.05.080
[2]	ZHENG S, CHEN B, QIU X, et al. Distribution and risk assessment of 82 pesticides in Jiulong River and estuary in South China[J]. Chemosphere, 2016, 144: 1177-1192. doi: 10.1016/j.chemosphere.2015.09.050
[3]	张志勇, 何丹军, 徐敦名, 等. 三唑磷在水中消解和残留动态研究[J]. 现代农药, 2005, 4(1): 25-27. doi: 10.3969/j.issn.1671-5284.2005.01.009
[4]	WANG Y, CHEN C, ZHAO X, et al. Assessing joint toxicity of four organophosphate and carbamate insecticides in common carp (Cyprinus carpio) using acetylcholinesterase activity as an endpoint[J]. Pestic Biochem Phys, 2015, 122: 81-85. doi: 10.1016/j.pestbp.2014.12.017
[5]	LIU L, ZHU B, GONG Y X, et al. Neurotoxic effect of triazophos on goldfish (Carassius auratus) and tissue specific antioxidant responses[J]. Ecotox Environ Saf, 2015, 116: 68-75. doi: 10.1016/j.ecoenv.2015.03.001
[6]	ZHU B, GONGY X, LIU L, et al. Toxic effects of triazophos on rare minnow (Gobiocypris rarus) embryos and larvae[J]. Chemosphere, 2014, 108: 46-54. doi: 10.1016/j.chemosphere.2014.03.036
[7]	YANG F W, FANG B, PANG G F, et al. Organophosphorus pesticide triazophos: a new endocrine disruptor chemical of hypothalamus-pituitary-adrenal axis[J]. Pestic Biochem Phys, 2019, 159: 91-97. doi: 10.1016/j.pestbp.2019.05.021
[8]	WU S, LI X, LIU X, et al. Joint toxic effects of triazophos and imidacloprid on zebrafish (Danio rerio)[J]. Environ Pollut, 2018, 235: 470-481. doi: 10.1016/j.envpol.2017.12.120
[9]	WANG G, SHAO J, WU M, et al. Effect of acute exposure of triazophos on histological structure and apoptosis of the brain and liver of zebrafish (Danio rerio)[J]. Ecotoxicol Environ Saf, 2019, 180: 646-655. doi: 10.1016/j.ecoenv.2019.05.053
[10]	WANG G, XIONG D, WU M, et al. Induction of time-and dose-dependent oxidative stress of triazophos to brain and liver in zebrafish (Danio rerio)[J]. Comp Biochem Physiol C, 2019, 228: 108640.
[11]	徐广春, 顾中言, 徐德进, 等. 五种常用杀虫剂对灰飞虱繁殖力的影响[J]. 植物保护学报, 2008, 35(4): 361-366. doi: 10.3321/j.issn:0577-7518.2008.04.014
[12]	孙伟, 张林宝, 胡莹, 等. 三唑磷作用下翡翠贻贝抑制性消减杂交文库构建与分析[J]. 中国环境科学, 2016, 36(12): 3807-3815. doi: 10.3969/j.issn.1000-6923.2016.12.036
[13]	JI C, WU H, WEI L, et al. Proteomic and metabolomic analysis reveal gender-specific responses of mussel Mytilus galloprovincialis to 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE 47)[J]. Aquat Toxicol, 2013, 140-141: 449-457. doi: 10.1016/j.aquatox.2013.07.009
[14]	JI C, LI F, WANG Q, et al. An integrated proteomic and metabolomic study on the gender-specific responses of mussels Mytilus galloprovincialis to tetrabromobisphenol A (TBBPA)[J]. Chemosphere, 2016, 144: 527-539. doi: 10.1016/j.chemosphere.2015.08.052
[15]	LUO L, ZHANG Q, KONG X, et al. Differential effects of Zn exposure on male and female oysters (Crassostrea angulata) as revealed by label-free quantitative proteomics: differential effects of Zn exposure on male and female oysters[J]. Environ Toxicol Chem, 2017, 36(10): 2602-2613. doi: 10.1002/etc.3797
[16]	LUO L, ZHANG Q, KONG X, et al. Differential effects of bisphenol A toxicity on oyster (Crassostrea angulata) gonads as revealed by label-free quantitative proteomics[J]. Chemosphere, 2017, 176: 305-314. doi: 10.1016/j.chemosphere.2017.02.146
[17]	ZHANG L, SUN W, ZHANG Z, et al. Gender-specific metabolic responses in gonad of mussel Perna viridis to triazophos[J]. Mar Pollut Bull, 2017, 123(1/2): 39-46.
[18]	CORTES-DIAZ M J A, RODRIGUEZ-FLORES J, CASTANEDA-PENALVO G, et al. Sublethal effects induced by captopril on Cyprinus carpio as determined by oxidative stress biomarkers[J]. Sci Total Environ, 2017, 605-606: 811-823. doi: 10.1016/j.scitotenv.2017.06.208
[19]	ELLMAN G L, COURTNEY K D, ANDRES V, et al. A new and rapid colorimetric determination of acetylcholinesterase activity[J]. Biochem Pharmacol, 1961, 7(2): 88-95. doi: 10.1016/0006-2952(61)90145-9
[20]	MORON M S, DEPIERRE J W, MANNERVIK B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver[J]. Biochim Biophys Acta, 1979, 582(1): 67-78. doi: 10.1016/0304-4165(79)90289-7
[21]	任加云, 潘鲁青, 苗晶晶. 苯并(a)芘和苯并(k)荧蒽混合物对栉孔扇贝毒理学指标的影响[J]. 环境科学学报, 2006, 26(7): 1150-1156.
[22]	AEBI H. Catalase in vitro[J]. Meth Enzymol, 1984, 105: 121-126. doi: 10.1016/S0076-6879(84)05016-3
[23]	OHKAWA H, OHISHI N, YAGI K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction[J]. Anal Biochem, 1979, 95(2): 351-358. doi: 10.1016/0003-2697(79)90738-3
[24]	FULTON M H, KEY P B. Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects[J]. Environ Toxicol Chem, 2001, 20(1): 37-45. doi: 10.1002/etc.5620200104
[25]	DETRA R L, COLLINS W J. The relationship of parathion concentration, exposure time, cholinesterase inhibition and symptoms of toxicity in midge larvae (Chironomidae: Diptera)[J]. Environ Toxicol Chem, 1991, 10: 1089-1095. doi: 10.1002/etc.5620100814
[26]	BINELLI A, RICCIARDI F, RIVA C, et al. New evidences for old biomarkers: effects of several xenobiotics on EROD and AChE activities in zebra mussel (Dreissena polymorpha)[J]. Chemosphere, 2006, 62(4): 510-519. doi: 10.1016/j.chemosphere.2005.06.033
[27]	ALVES S, SEVERINO P, IBBOTSON D, et al. Effects of furadan in the brown mussel Perna perna and in the mangrove oyster Crassostrea rhizophorae[J]. Mar Environ Res, 2002, 54(3/4/5): 241-245. doi: 10.1016/S0141-1136(02)00138-1
[28]	LIONETTO M G, CARICATO R, GIORDANO M E, et al. Integrated use of biomarkers (acetylcholinesterase and antioxidant enzymes activities) in Mytillus galloprovincialis and Mullus barbatus in an Italian coastal marine area[J]. Mar Pollut Bull, 2003, 46(3): 324-330. doi: 10.1016/S0025-326X(02)00403-4
[29]	CHOI J Y, YU J, YANG D B, et al. Acetylthiocholine (ATC) cleaving cholinesterase (ChE) activity as a potential biomarker of pesticide exposure in the Manila clam, Ruditapes philippinarum, of Korea[J]. Mar Environ Res, 2011, 71(3): 162-168. doi: 10.1016/j.marenvres.2010.12.007
[30]	VALDEZ DOMINGOS F X, AZEVEDO M, SILVA M D, et al. Multibiomarker assessment of three Brazilian estuaries using oysters as bioindicators[J]. Environ Res, 2007, 105(3): 350-363. doi: 10.1016/j.envres.2007.06.003
[31]	DORAN W J, COPE W G, RADA R G, et al. Acetylcholinesterase inhibition in the threeridge mussel (Amblema plicata) by chlorpyrifos: implications for biomonitoring[J]. Ecotoxicol Environ Saf, 2001, 49(1): 91-98. doi: 10.1006/eesa.2000.2036
[32]	余向阳, 赵于丁, 王冬兰, 等. 毒死蜱和三唑磷对斑马鱼头部AChE活性影响及在鱼体内的富集[J]. 农业环境科学学报, 2008, 27(6): 2452-2455. doi: 10.3321/j.issn:1672-2043.2008.06.058
[33]	SINGH S, TIWARI R K, PANDEY R S. Acute toxicity evaluation of triazophos, deltamethrin and their combination on earthworm, Eudrilus eugeniae and its impact on AChE activity[J]. J Chem Ecol, 2019, 35(6): 563-575. doi: 10.1080/02757540.2019.1600679
[34]	刘海涛, 李兵, 王国宝, 等. 辛硫磷对家蚕雌雄个体的毒力比较[J]. 江苏农业科学, 2010, 6: 390-392. doi: 10.3969/j.issn.1002-1302.2010.02.160
[35]	刘海涛. 家蚕对有机磷农药抗性的性别差异研究[D]. 苏州: 苏州大学, 2010: 47-46.
[36]	郑波, 石年, 李龙, 等. 螨胺磷在不同性别大鼠中代谢差异初探[J]. 同济医科大学学报, 2000, 29(2): 127-129.
[37]	MAHARAJAN K, MUTHULAKSHMI S, NATARAJ B, et al. Toxicity assessment of pyriproxyfen in vertebrate model zebrafish embryos (Danio rerio): a multi biomarker study[J]. Aquat Toxicol, 2018, 196: 132-145. doi: 10.1016/j.aquatox.2018.01.010
[38]	ZHANG J F, WANG X R, GUO H Y, et al. Effects of water soluble fractions of diesel oil on the antioxidant defenses of the goldfish, Carassius auratus[J]. Ecotoxicol Environ Saf, 2004, 58(1): 110-116. doi: 10.1016/j.ecoenv.2003.08.025
[39]	钟碧瑾, 姚丹, 刘娟娟, 等. 两种有机磷农药对沼水蛙蝌蚪抗氧化系统及MDA浓度的影响[J]. 福建师范大学学报(自然科学版), 2009, 25(2): 91-96.
[40]	XIU M, PAN L, JIN Q. Toxic effects upon exposure to polycyclic aromatic hydrocarbon (chrysene) in scallop Chlamys farreri during the reproduction period[J]. Environ Toxicol Phar, 2016, 44: 75-83. doi: 10.1016/j.etap.2016.04.001
[41]	吉成龙. 典型溴系阻燃剂对紫贻贝毒理效应的组学研究[D]. 烟台: 中国科学院烟台海岸带研究所, 2014: 107-109.